DiaPlexQ™ Novel Coronavirus (2019-nCoV) Detection Kit CE-IVD
- Details
- Parent Category: Каталог Продуктов
- Category: Молекулярная диагностика ПЦР(PCR)
Real-Time OneStep RT-PCR based assay system for detection of 2019-nCoV
CE-IVD
DiaPlexQ™ Novel Coronavirus(2019-nCov) Detection Kit is CE-IVD reagent. It is screening kit to detect Novel Corona Virus as 2019-nCoV from isolated total RNA. Basic principal of this kit is Real-time PCR method, which is able to detect specific target gene into total RNA. It provides every necessary contents for PCR reaction, Therefore customer don’t need to purchase additional reagent. One step RT-PCR contents are progressively apply for one tube RT(Reverse Transcription) reaction and PCR amplification. You can monitoring the nucleic acid amplification result based on real time condition through the amplification plot. The Control Template (2019-nCov) is provide as positive control to assist the clinical sample data comparison analysis.
Contens
| Components | | SQD52-K020 | SQD52-K100 | |
|---|---|---|---|---|
| 2X OneStep qRT-PCR Buffer (2019-nCoV) |
200 ul x 1 ea | 1.0 ml x 1ea | ||
| OneStep qRT-PCR Enzyme mix (2019-nCoV) |
40 ul x 1 ea | 200 ul x 1 ea | ||
| Primer & Probe Mixture (2019-nCoV) |
60 ul x 1 ea | 300 ul x 1 ea | ||
| Control Template (2019-nCoV) |
20 ul x 1 ea | 100 ul x 1 ea | ||
| RNase Free Water |
200 ul x 1 ea | 1.0 ml x 1 ea |
Specification
| Detection target | 2019-nCoV ( COVID-19 ) |
| Target region | - ORF1a - N gene |
| Detection technology | Real-Time OneStep RT-qPCR |
| Specimen type | - Nasopharyngeal swab - Oropharyngeal swab - Sputum |
| Compatible instruments* | - Applied Biosystems™ 7500 Real-Time PCR Instrument System - Applied Biosystems™ 7500 Fast Real-Time PCR Instrument System - Bio-Rad CFX96™ System |
| PCR running time | ~ 120 mins |
Features
- Simple & Rapid detection system: OneStep Multiplex RT-qPCR based detection
- HotStart PCR: high-specificity
- Reliable system: automatic PCR control (not Internal control)
- Easy-to-use master mix: just adding template and Primer/Probe Mix
- Positive control included (Plasmid)
Experimental
| Types | 5' Fluorophore | 3' Quencher |
|---|---|---|
| N gene |
FAM |
BHQ1 |
| orf1a |
VIC/JOE* | BHQ1 |
| PCR control | Texas Red/Cal Fluor Red 610* | BHQ2 |
Process
Sample collection
- Sample collection according to sample type
Nucleic acid isolation
- Manual Method
- Auto extraction Method
Multiplex qPCR
DiaPlexQ ™Novel Coronavirus (2019-nCoV) Detection Kit
Data analysis
- Use of software For each instrument